ALIOS·PES

Bioprinter

The BP-X3 3D bioprinter

Up to 30 technical replicates from a single T150 flask — the throughput your research demands.

  • µL-scale dispensing accuracy, no matter the bioink.
  • Software-enabled throughput across multi-well plates.
  • Output-oriented: up to 30 technical replicates per single T150 flask.
  • Simple to use. Train a new user in two hours, with no prior experience.
  • Born out of doctoral research at the University of Birmingham.
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The BP-X3 3D bioprinter, with a multi-well plate on the print bed, inside a laminar flow cabinet

Example applications

Built for reproducible 3D in-vitro work

Disease modelling

Build multi-cell-type constructs and co-cultures that hold their geometry through long maturation.

Compound screening

Generate matched technical replicates across a plate for dose–response and phenotype studies.

Biomaterial development

Print the bioinks your protocol already uses — the suspended process is biomaterial-agnostic.

Validated applications

All presented applications have been produced with a development version of the Polysaccharide–Collagen I reagent kit.

A single 3D-bioprinted technical replicate, shown from above and in profile

The technical replicate

Available for 3D cell culture on its own, or as a layer in a multi-layer co-culture in-vitro 3D tissue model. Compatible with long-timeline studies of 21 days and over.

Scale bar: 5 mm.

Hoechst-stained cross-sections of a multi-layer bioprinted construct

Multi-layer integration

Integration of distinct layers in a single technical replicate for complex tissue in-vitro modelling. Each layer is seeded at 0.5×106 cells/ml (bottom layers), 1.0×106 cells/ml (middle layer) and 1.5×106 cells/ml (top layer).

Images show cross-sections stained with Hoechst 33342 nuclear stain. Scale bar: 3 mm.

Fluorescent and second harmonic generation imaging of a 3D skin model at day 3 and day 21

Label-free ECM characterisation

Label-free characterisation for insight with minimal processing. Fluorescent (green) and second harmonic generation (B&W) imaging showcasing ECM deposition at the dermal–epidermal junction (white arrow) of full-thickness in-vitro 3D skin models after 21 days of culture, compared to 3 days. Models composed of human epidermal keratinocytes, human dermal fibroblasts and adipose-derived stem cells.

Legend: E, D and H — epidermis, dermis and hypodermis, respectively. Scale bar: 1 mm.

Immunohistochemical staining for alpha-smooth muscle actin in a 3D in-vitro model

Immunohistochemistry in bulk sections

Avoiding the harshness of standard microtomy prep, local protein expression can be resolved within a bulk 800 µm section of a bioprinted construct, only fixed with 4% Paraformaldehyde. Here, α-smooth muscle actin (SMA) is visualised in a full-thickness in-vitro 3D model of skin, comprised of a hypodermis, a dermis and an epidermis after 21 days' culture.

Scale bar: 1 mm.

Section of an RNAseq heat map comparing transcription profiles at day 3 and day 21

Full-transcriptome RNAseq

Full-transcriptome RNAseq for comprehensive analysis of in-vitro phenotypes. The image presents a section of the heat map from a study on the development of in-vitro skin in full-thickness 3D models, comparing bulk model transcription profiles after 3 days and 21 days of culture.